At the Bench: A Laboratory NavigatorCSHL Press, 2005 - 465 páginas From the Publisher: "A research laboratory filled with competent, busy people entirely familiar with its arcane customs and practices is a daunting place for newcomers. Kathy Barker knows this world. She was a technician, an undergraduate, then a graduate student at the University of Massachusetts, and as a postdoctoral fellow and assistant professor at Rockefeller University, she was a mentor to grad students, physicians in training, technicians, and research nurses. From this rich experience, she has written At the Bench, a unique handbook for living and working in the laboratory. Much more than a simple primer or lab manual, this book is an essential aid to understanding: how research groups work at a human level-and how to fit in; what equipment is essential, and how to use it properly; how to get started and get organized; how to set up an experiment; how to handle and use data and reference sources; how to present yourself and your results-in print and in person. Wise, light-hearted, but thoroughly practical, Dr. Barker offers advice, moral support, social etiquette, and professional reassurance along with assume-nothing, step-by-step instructions for those basic but vital laboratory procedures that experienced investigators know-but may not realize novices don't. If you are a graduate student, a physician with research intentions, or a laboratory technician, this book is indispensable. If you have to manage or mentor such people, giving a copy to each of them will greatly improve your life, and theirs." |
Contenido
| 21 | |
| 43 | |
Plotting a Course | 67 |
Presenting Yourself and Your Data | 101 |
Navigating | 129 |
Storage and Disposal | 165 |
Working without Contamination | 187 |
Eukaryotic Cell Culture | 207 |
Bacteria | 247 |
DNA RNA and Protein | 279 |
Radioactivity | 313 |
Centrifugation | 347 |
Microscopy | 405 |
Glossary | 433 |
Index | 455 |
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Términos y frases comunes
acrylamide agarose aliquots antibiotics antibody aseptic assay autoclaved autoradiography bacteria beaker biohazard biosafety cabinet bottle buffer cell lines centrifuge chemical Cold Spring Harbor colonies concentration container counting cylinder detergent dilution discard disposal EDTA electrode electrophoresis enzyme equipment ethanol example experiment film filter flask fluorescence freezer freezing fume hood Fyrite gene glass gloves growth H₂O hazardous incubator isolation isotopes lab members lab notebook labeled light liquid nitrogen manual material medium membrane mg/ml microfuge tube microscope minutes molecular molecule mycoplasma NaCl organisms paper Pasteur pipette pellet pH meter phenol pipette pipettor plasmid plastic plate protein protocol radioactive reagents remove require restriction enzymes room temperature rotor safety sample scintillation serum slide solvents spin stain sterile stir bar stock solution storage stored supernatant sure tank tion tissue usually vacuum vials volume wash waste µg/ml
Pasajes populares
Página 280 - Harlow E. and Lane D. 1988. Antibodies: A laboratory manual Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Página 69 - I have often had cause to feel that my hands are cleverer than my head. That is a crude way of characterizing the dialectics of experimentation. When it is going well, it is like a quiet conversation with Nature. One asks a question and gets an answer; then one asks the next question, and gets the next answer. An experiment is a device to make Nature speak intelligibly. After that one has only to listen.
Página 280 - Ausubel, FM, Brent, R., Kingston, RE, Moore, DD, Seidman, JG, Smith, JA, and Struhl, K. (1999) Current Protocols in Molecular Biology, John Wiley & Sons, New York.
Página 247 - A Short Course in Bacterial Genetics. A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria.
Página 410 - The lowest temperature at which a liquid gives off sufficient vapor to form an ignitable mixture with air near the surface of the liquid is called the flash point.
Página 116 - Ethidium bromide is a powerful mutagen and is moderately toxic. Gloves should be worn when working with solutions that contain this dye. After use, these solutions should be decontaminated by one of the methods described in Appendix E.
Página 116 - Phenylmethylsulfonyl fluoride (PMSF) is extremely destructive to the mucous membranes of the respiratory tract, the eyes, and the skin. It may be fatal if inhaled, swallowed, or absorbed through the skin. In case of contact, immediately flush eyes or skin with copious amounts of water and discard contaminated clothing.
Página 155 - Using Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
Página 116 - Stir vigorously on a magnetic stirrer. Adjust the pH to 8.0 with NaOH (-20 g of NaOH pellets <!>). Dispense into aliquots and sterilize by autoclaving.
Página 118 - ... at -20°C. As needed, remove the phenol from the freezer, allow it to warm to room temperature, and then melt it at 68°C. Add hydroxyquinoline to a final concentration of 0.1%. This compound is an antioxidant, a partial inhibitor of RNase, and a weak chelator of metal ions (Kirby 1956). In addition, its yellow color provides a convenient way to identify the organic phase. 2. To the melted phenol, add an equal volume of buffer (usually 0.5 M Tris-Cl [pH 8.0] at room temperature).