New Approaches for the Generation and Analysis of Microbial Typing DataL. Dijkshoorn, K.J. Towner, Mark J Struelens Elsevier, 2001 M07 10 - 370 páginas Rapid molecular identification and typing of micro-organisms is extremely important in efforts to monitor the geographical spread of virulent, epidemic or antibiotic-resistant pathogens. It has become a mainstay of integrated hospital infection control service. In addition, numerous industrial and biotechnological applications require the study of the diversity of organisms. Conventional phenotypic identification and typing methods have long been the mainstay of microbial population and epidemiological studies, but such methods often lack adequate discrimination and their use is normally confined to the group of organisms for which they were originally devised. Molecular fingerprinting methods have flourished in recent years and many of these new methods can be applied to numerous different organisms for a variety of purposes. Standardisation of these methods is vitally important. In addition, the generation of large numbers of complex fingerprint profiles requires that a computer-assisted strategy is used for the formation and analysis of databases. The purpose of this book is to describe the best fingerprinting methods that are currently available and the computer-assisted strategies that can be used for analysis and exchange of data between laboratories.
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Contenido
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Chapter 3 SettingUp Intra and InterLaboratory Databases of Electrophoretic Profiles | 47 |
Chapter 4 Fingerprinting of Microorganisms by Protein and Lipopolysaccharide SDSPAGE ... | 77 |
Chapter 5 rRNA Gene Restriction Pattern Determination Ribotyping and Computer Interpretation ... | 107 |
Chapter 6 Generation and Analysis of RAPD Fingerprinting Profiles | 135 |
Chapter 7 Analysis of Microbial Genomic Macrorestriction Patterns by Pulsed Field Gel Electrophoresis PFGE Typing ... | 159 |
Chapter 8 Selective Restriction Fragment Amplification by AFLP TM | 177 |
Chapter 9 Application and Analysis of ARDRA Patterns in Bacterial Identification Taxonomy and Phylogeny | 211 |
Chapter 10 Insertion Sequence IS Typing and Oligotyping | 249 |
Chapter 11 Molecular Characterisation of Microbial Communities Based on 16S rRNA Sequence Diversity ... | 267 |
Chapter 12 From Multilocus Enzyme Electrophoresis to Multilocus Sequence Typing | 299 |
| 351 | |
| 353 | |
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New Approaches for the Generation and Analysis of Microbial Typing Data Lenie Dijkshoorn,K. J. Towner,Marc Struelens Sin vista previa disponible - 2001 |
Términos y frases comunes
16S rRNA gene Acinetobacter addition AFLP amplicons amplified analysis Applied approach ARDRA associated bacterial Bacteriology bands buffer calculated cell characters Clinical Microbiology clone cluster coefficient coli combination compared comparison containing correlation database described detection determine differentiation disease diversity electrophoresis Environmental enzyme epidemiological et al example extracts fingerprinting followed fragments gel electrophoresis genetic genomic genotypic Grimont hybridisation identification important Infection International isolates Journal of Clinical laboratories lanes length markers methods microbial molecular nucleic acids objects obtained organisms outbreak patterns performed PFGE phylogenetic polymorphism population position possible preparation primers probe procedure profiles protein RAPD reference reproducibility Research restriction ribosomal ribotyping samples selective separation sequence similarity single species standard step strains structure Systematic Table taxonomic techniques tion tuberculosis typing United values variation
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