Authentication Of Chinese Medicinal Materials By Dna TechnologyWorld Scientific, 2002 M06 19 - 244 páginas Traditional Chinese medicine (TCM) has been used for centuries in the Orient to relieve or treat diseases. Its efficacy is now attracting worldwide attention. Chinese medicinal materials are traditionally identified by their organoleptic characteristics, such as the texture or the odor. To ensure consistency of quality and avoid misuse, reliable programs are urgently needed for authentication. Proper authentication also assists in the detection and conservation of endangered species. DNA molecules offer a more definitive means of authentication, as genetic composition is unique for each taxon and is less affected by age, physiological conditions and environmental factors. This book is the first of its kind to introduce and evaluate the molecular techniques that have been developed to authenticate Chinese medicinal materials.The contents of the book are divided into four parts. The first part reviews the current status of molecular authentication of Chinese medicinal materials. The second part describes the equipment and materials required for the work. In the third part, researchers in the field present the principles and step-by-step protocols for a wide spectrum of techniques used in the molecular authentication of TCM. The last part provides an overview of this field. |
Contenido
1 | |
2 Methodology and Equipment for General Molecular Techniques Forrest ChungFai Yau and FaiNgor Ngan | 25 |
3 Authentication of Chinese Medicinal Materials by Polymerase Chain Reaction Using Arbitrarily Chosen Primers Hui Cao Paul PuiHay But Fai Ngor ... | 43 |
4 Authentication of the Components from a Mixture of Herbal Materials KurTa Cheng | 69 |
5 Authentication of Chinese Medicinal Materials by Sequence Characterised Amplified Region Jun Wang WaiYan Ha Forrest ChungFai Yau Paul Pui... | 85 |
6 Differentiation of Panax ginseng from P quinquefolius by Direct Amplification of Length Polymorphisms WaiYan Ha Forrest ChungFai Yau Jun ... | 97 |
7 Differentiation of Panax ginseng from P quinquefolius by Amplified Fragment Length Polymorphism WaiYan Ha Forrest ChungFai Yau PangChui... | 109 |
8 Authentication of Animal Medicinal Materials by DNA Molecular Markers Yiquan Wang and Kaiya Zhou | 125 |
9 Authentication of Medicinal Dendrobium Species by the Internal Transcribed Spacer of Ribosomal DNA David TaiWai Lau PangChui Shaw and P... | 155 |
10 Authentication by DNA Sequencing of 5s rRNA and trnK Genes Hajime Mizukami | 169 |
11 Authentication Based on 18S Ribosomal RNA Gene and matK Gene Sequences Katsuko Komatsu | 193 |
12 Outlook PangChui Shaw Jun Wang Paul PuiHay But and Forrest ChungFai Yau | 213 |
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Otras ediciones - Ver todas
Authentication of Chinese Medicinal Materials by DNA Technology Pang-Chui Shaw,Paul P. H. But,Jun Wang Vista previa limitada - 2002 |
Authentication of Chinese Medicinal Materials by DNA Technology Pang-Chui Shaw,J. Wang,Paul P. H. But Vista previa limitada - 2002 |
Authentication of Chinese Medicinal Materials by DNA Technology Pang-Chui Shaw,J. Wang,Paul P. H. But Sin vista previa disponible - 2002 |
Términos y frases comunes
18S rRNA gene adulterants AFLP agarose gel amplified polymorphic DNA Angelica acutiloba annealing AP-PCR Applied Genetics Atractylodes centrifuge China Chinese medicinal materials chuanxiong crude drugs CTAB DALP denaturing Dendrobium species detected differentiate DNA extraction DNA fingerprinting DNA fragments DNA sequence dNTPs dried rhizomes ethidium bromide fragment length polymorphism genomic genomic DNA ginseng drugs Guangxi herba dendrobii herbal Hong Kong hybridisation identification intraspecific japonicus Jiangsu Journal kudidan lane matK gene method MgCl2 minisatellite Mizukami molecular markers Nucleic Acids Research nucleotide nucleotide sequences original animals panacis Panax ginseng PCR amplification PCR product PCR-RFLP Pharmaceutical Bulletin Planta Medica plants polymerase chain reaction polymorphic polymorphic bands primer Procedures quinquefolius radix RAPD RAPD analysis rDNA Reagents refer to Section restriction fragment RFLP rhizome root samples SCAR seahorse shi hu spacer region substitutes Taq DNA polymerase technique template DNA Theoretical and Applied Thermocycler Tris-HCl trnK turtle shell U/µl Wang Zhou