Authentication Of Chinese Medicinal Materials By Dna Technology

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World Scientific, 2002 M06 19 - 244 páginas
Traditional Chinese medicine (TCM) has been used for centuries in the Orient to relieve or treat diseases. Its efficacy is now attracting worldwide attention. Chinese medicinal materials are traditionally identified by their organoleptic characteristics, such as the texture or the odor. To ensure consistency of quality and avoid misuse, reliable programs are urgently needed for authentication. Proper authentication also assists in the detection and conservation of endangered species. DNA molecules offer a more definitive means of authentication, as genetic composition is unique for each taxon and is less affected by age, physiological conditions and environmental factors. This book is the first of its kind to introduce and evaluate the molecular techniques that have been developed to authenticate Chinese medicinal materials.The contents of the book are divided into four parts. The first part reviews the current status of molecular authentication of Chinese medicinal materials. The second part describes the equipment and materials required for the work. In the third part, researchers in the field present the principles and step-by-step protocols for a wide spectrum of techniques used in the molecular authentication of TCM. The last part provides an overview of this field.
 

Contenido

1 Molecular Markers in Chinese Medicinal Materials PangChui Shaw FaiNgor Ngan Paul PuiHay But and Jun Wang
1
2 Methodology and Equipment for General Molecular Techniques Forrest ChungFai Yau and FaiNgor Ngan
25
3 Authentication of Chinese Medicinal Materials by Polymerase Chain Reaction Using Arbitrarily Chosen Primers Hui Cao Paul PuiHay But Fai Ngor ...
43
4 Authentication of the Components from a Mixture of Herbal Materials KurTa Cheng
69
5 Authentication of Chinese Medicinal Materials by Sequence Characterised Amplified Region Jun Wang WaiYan Ha Forrest ChungFai Yau Paul Pui...
85
6 Differentiation of Panax ginseng from P quinquefolius by Direct Amplification of Length Polymorphisms WaiYan Ha Forrest ChungFai Yau Jun ...
97
7 Differentiation of Panax ginseng from P quinquefolius by Amplified Fragment Length Polymorphism WaiYan Ha Forrest ChungFai Yau PangChui...
109
8 Authentication of Animal Medicinal Materials by DNA Molecular Markers Yiquan Wang and Kaiya Zhou
125
9 Authentication of Medicinal Dendrobium Species by the Internal Transcribed Spacer of Ribosomal DNA David TaiWai Lau PangChui Shaw and P...
155
10 Authentication by DNA Sequencing of 5s rRNA and trnK Genes Hajime Mizukami
169
11 Authentication Based on 18S Ribosomal RNA Gene and matK Gene Sequences Katsuko Komatsu
193
12 Outlook PangChui Shaw Jun Wang Paul PuiHay But and Forrest ChungFai Yau
213
Index
225
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