Laboratory Handbook for the Fractionation of Natural ExtractsSpringer Science & Business Media, 2012 M12 6 - 199 páginas This laboratory manual will be welcomed by all research scientists involved in the extraction, fractionation and isolation of compounds from natural materials, especially those working with plants. The book is clear and concise, and features practical exercises to illustrate the techniques described in every chapter. It will provide an invaluable research reference tool for those scientists investigating the potential benefits of ethnomedicine and the properties of chemicals isolated from natural flora. |
Contenido
1 | |
Important concepts | 14 |
2 | 20 |
Crude fractionation procedures | 54 |
Chromatographic procedures using columns and liquidliquid | 82 |
Layer chromatography | 104 |
1 | 113 |
1 | 127 |
General methods | 140 |
Practical exercises in chemical screening and fractionation | 154 |
Appendix A Recipes for chemical test reagents | 184 |
Otras ediciones - Ver todas
Laboratory Handbook for the Fractionation of Natural Extracts Peter Houghton,Amala Raman Sin vista previa disponible - 1998 |
Laboratory Handbook for the Fractionation of Natural Extracts Peter Houghton,Amala Raman Sin vista previa disponible - 2012 |
Términos y frases comunes
absorbance acetone achieved adsorption aglycone alcohol aliquots alkaloids analytical TLC anthraquinones applied aqueous extract bands biological boiling Buchner caffeine cardenolides cellulose Centrifuge chloride chloroform chlorophyll clean-up colour column chromatography components concentration constituents containing detection dichloromethane dilute dissolved distillation eluant eluted ethyl acetate evaporating dish Figure filter filtration Fixed oils flask fluid fractionation glycosides heating hexane HPLC ion-exchange isolated layer less polar light petroleum methanol method mixture mobile phase molecules natural products non-polar obtained partition phenolic phenolic compounds plant material polar solvents polyphenols polyvinylpolypyrrolidone pores powdered precipitate preparative present pressure procedures proteins range removed resin reverse-phase Rotary evaporator sample Sephadex silica gel sodium solubility solution solvent extraction spray reagent stationary phase Step substances sugars sulphuric acid supernatant technique temperature thin-layer TLC plate tube types usually UV light vacuum volatile oils volume water bath zones