Laboratory Handbook for the Fractionation of Natural ExtractsSpringer Science & Business Media, 2012 M12 6 - 199 páginas This laboratory manual will be welcomed by all research scientists involved in the extraction, fractionation and isolation of compounds from natural materials, especially those working with plants. The book is clear and concise, and features practical exercises to illustrate the techniques described in every chapter. It will provide an invaluable research reference tool for those scientists investigating the potential benefits of ethnomedicine and the properties of chemicals isolated from natural flora. |
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Página 3
... alcohol content , colour and astringency found in wine . The amount of a particular ' active ingredient ' in an organism can there- fore vary considerably due to one or more of the above factors and this obviously makes a difference ...
... alcohol content , colour and astringency found in wine . The amount of a particular ' active ingredient ' in an organism can there- fore vary considerably due to one or more of the above factors and this obviously makes a difference ...
Página 4
... alcohol . These have little history of scientific testing for efficacy or safety . Those who use such preparations justify their actions on the grounds of tradi- tion and anecdotal evidence . Two important points should be noted in the ...
... alcohol . These have little history of scientific testing for efficacy or safety . Those who use such preparations justify their actions on the grounds of tradi- tion and anecdotal evidence . Two important points should be noted in the ...
Página 12
... treating the original compound with various reagents , e.g. to produce esters from acids or alcohols or to methylate oxygen or nitrogen atoms around the molecule . Step 11 Once an interesting activity has been found then 12 Introduction.
... treating the original compound with various reagents , e.g. to produce esters from acids or alcohols or to methylate oxygen or nitrogen atoms around the molecule . Step 11 Once an interesting activity has been found then 12 Introduction.
Página 18
... alcohols if acids are present in the extract . The use of ammonia solution is to be avoided if material is under investigation for the presence of alkaloids , since the nitrogen from ammonia is often incorporated into molecules to yield ...
... alcohols if acids are present in the extract . The use of ammonia solution is to be avoided if material is under investigation for the presence of alkaloids , since the nitrogen from ammonia is often incorporated into molecules to yield ...
Página 40
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Contenido
1 | |
14 | |
2 | 20 |
Crude fractionation procedures | 54 |
Chromatographic procedures using columns and liquidliquid | 82 |
Layer chromatography | 104 |
1 | 113 |
1 | 127 |
General methods | 140 |
Practical exercises in chemical screening and fractionation | 154 |
Appendix A Recipes for chemical test reagents | 184 |
Otras ediciones - Ver todas
Laboratory Handbook for the Fractionation of Natural Extracts Peter Houghton,Amala Raman Sin vista previa disponible - 1998 |
Laboratory Handbook for the Fractionation of Natural Extracts Peter Houghton,Amala Raman Sin vista previa disponible - 2012 |
Términos y frases comunes
absorbance acetone achieved adsorption aglycone alcohol aliquots alkaloids analytical TLC anthraquinones applied aqueous extract bands biological boiling Buchner caffeine cardenolides cellulose Centrifuge chloride chloroform chlorophyll clean-up colour column chromatography components concentration constituents containing detection dichloromethane dilute dissolved distillation eluant eluted ethyl acetate evaporating dish Figure filter filtration Fixed oils flask fluid fractionation glycosides heating hexane HPLC ion-exchange isolated layer less polar light petroleum methanol method mixture mobile phase molecules natural products non-polar obtained partition phenolic phenolic compounds plant material polar solvents polyphenols polyvinylpolypyrrolidone pores powdered precipitate preparative present pressure procedures proteins range removed resin reverse-phase Rotary evaporator sample Sephadex silica gel sodium solubility solution solvent extraction spray reagent stationary phase Step substances sugars sulphuric acid supernatant technique temperature thin-layer TLC plate tube types usually UV light vacuum volatile oils volume water bath zones