Physicochemical Methods in the Study of Biomembranes

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Herwig J. Hilderson, Gregory B. Ralston
Springer Science & Business Media, 2013 M11 11 - 509 páginas
In mammalian cells many physiological processes rely on the dynamics of the organization of lipids and proteins in biological membranes. The topics in this volume deal with physicochemical methods in the study of biomembranes. Some of them have a long and respectable history in the study of soluble proteins and have only recently been applied to the study of membranes. Some have tradi tionally been applied to studies of model systems of lipids of well-defined com position, as well as to intact membranes. Other methods, by their very nature, apply to organized bilayers comprised of both protein and lipid. Van Meer and van Genderen provide us with an introduction to the field (Chapter I). From their personal perspective regarding the distribution, trans port, and sorting of membrane lipids, they formulate a number of biologically relevant questions and show that the physicochemical methods described in this book may contribute in great measure to solving these issues. The methods of analytical ultracentrifugation have served faithfully for 60 years in the study of water-soluble proteins. The use of detergent extraction of membrane proteins, and the manipulation of density with H20/D20 mixtures, has extended this technique to the study of proteins, and in particular their interactions, from biological membranes. As described by Morris and Ralston in Chapter 2, this technique can be used to determine a number of important properties of proteins.
 

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Contenido

Chapter I
1
How Do Lipids Get Where They Are?
10
Final Remarks
19
Chapter 2
25
Basic Concepts
28
Instrumentation
34
Sedimentation Velocity
40
Diffusion
56
Chapter 7
247
Causes of Transmembrane Chemical Shift Differences
259
NMR Studies of Slow Membrane Transport Processes
269
NMR Studies of Fast Membrane Transport Processes
278
Compartmental Discrimination Using Diffusion Rates
301
Concluding Remarks 3 12
312
98
323
Chapter 8
329

References
74
Chapter 3
83
Examples of the Use of Monomolecular Layers
93
Perspective
112
Chapter 4
121
Dynamic Properties of Membrane Phase Transition
138
Calorimetric Studies of LipidProtein Interactions
150
Chapter 5
161
Factors Determining Erythrocyte Deformability
168
Use of the Ektacytometer for Detection and Analysis
177
Experimental Alteration of Membrane Mechanical Properties
188
References
196
Chapter 6
205
Electrostatic Origin of Lipid Selectivity
217
Line Shape Effects of SolvationtoFluid Exchange
224
Novel Evidence for the TwoSite Exchange Model
235
Secondary Structures of Peptide Model Compounds
342
References 3 13
351
References
357
102
358
107
383
References
395
111
402
Chapter 10
405
References
441
Chapter 11
451
Monolayers and Langmuir
480
121
486
122
493
149
500
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