Physicochemical Methods in the Study of BiomembranesHerwig J. Hilderson, Gregory B. Ralston Springer Science & Business Media, 2013 M11 11 - 509 páginas In mammalian cells many physiological processes rely on the dynamics of the organization of lipids and proteins in biological membranes. The topics in this volume deal with physicochemical methods in the study of biomembranes. Some of them have a long and respectable history in the study of soluble proteins and have only recently been applied to the study of membranes. Some have tradi tionally been applied to studies of model systems of lipids of well-defined com position, as well as to intact membranes. Other methods, by their very nature, apply to organized bilayers comprised of both protein and lipid. Van Meer and van Genderen provide us with an introduction to the field (Chapter I). From their personal perspective regarding the distribution, trans port, and sorting of membrane lipids, they formulate a number of biologically relevant questions and show that the physicochemical methods described in this book may contribute in great measure to solving these issues. The methods of analytical ultracentrifugation have served faithfully for 60 years in the study of water-soluble proteins. The use of detergent extraction of membrane proteins, and the manipulation of density with H20/D20 mixtures, has extended this technique to the study of proteins, and in particular their interactions, from biological membranes. As described by Morris and Ralston in Chapter 2, this technique can be used to determine a number of important properties of proteins. |
Contenido
Chapter 7 | 247 |
Causes of Transmembrane Chemical Shift Differences | 259 |
NMR Studies of Slow Membrane Transport Processes | 269 |
NMR Studies of Fast Membrane Transport Processes | 278 |
Compartmental Discrimination Using Diffusion Rates | 301 |
Concluding Remarks | 312 |
Determination of Soluble and Membrane Protein Structure | 329 |
Introduction | 330 |
4 | 63 |
8 | 73 |
Chapter 3 | 83 |
6 | 89 |
Perspective | 112 |
Chapter 4 | 121 |
Dynamic Properties of Membrane Phase Transition | 138 |
Calorimetric Studies of LipidProtein Interactions | 150 |
Chapter 5 | 161 |
6 | 177 |
8 | 188 |
References | 196 |
Chapter 6 | 205 |
Electrostatic Origin of Lipid Selectivity | 217 |
Line Shape Effects of SolvationtoFluid Exchange | 224 |
Novel Evidence for the TwoSite Exchange Model | 235 |
Secondary Structures of Peptide Model Compounds | 342 |
Dipole Orientation | 349 |
References | 357 |
Chapter 9 | 363 |
Amino Acid Side Chain Structure | 379 |
HydrogenDeuterium Exchange | 383 |
References | 395 |
Chapter 10 | 405 |
Determination of Protein Secondary Structures by Pattern | 435 |
References | 441 |
Chapter 11 | 451 |
Monolayers and Langmuir | 480 |
493 | |
500 | |
Otras ediciones - Ver todas
Physicochemical Methods in the Study of Biomembranes Herwig J. Hilderson,Gregory B. Ralston Sin vista previa disponible - 1994 |
Physicochemical Methods in the Study of Biomembranes Herwig J. Hilderson,Gregory B. Ralston Sin vista previa disponible - 2012 |
Physicochemical Methods in the Study of Biomembranes Herwig J Hilderson,Gregory B Ralston Sin vista previa disponible - 2014 |
Términos y frases comunes
acid Acta amide analysis analytical ultracentrifuge associated bacteriorhodopsin Biltonen binding Biochemistry Biochim Biol biological membranes Biophys boundary cardiolipin chain Chem chemical shift cholesterol complex components concentration constant cytochrome deformability Demel detergent determined diffraction diffusion DMPC DMPC-DSPC domains DPPC effects enzyme Equation erythrocytes exchange experiments Figure film fraction frequency function glycosphingolipids gradient heat capacity heterogeneity Horváth human erythrocytes hydration hydrogen infrared integral membrane proteins interactions interface intracellular kinetics Kuchel line shape lipid bilayers lipid-protein magnetic measured membrane proteins method MLVs mN/m Mohandas molecular weight molecules monolayers myelin nonideality obtained optics parameters peptide phase transition phosphatidylcholine phospholipid plasma membrane protein/lipid proteolipid pulse Ralston ratio reaction red cells relaxation resonance sample Schachman secondary structure sedimentation equilibrium sedimentation velocity self-association shift solvation solvation lipids solvent specific spectra spectrin spectroscopy spectrum spin spin-labeled studies surface pressure techniques temperature tion titration transport vesicles viscosity Yphantis